Project Number: 5010-41000-135-04
Start Date: Dec 15, 2009
End Date: Jun 14, 2014
Fuel ethanol is not produced under aseptic conditions, and acute bacterial infections occur unpredictably leading to "stuck" fermentations. We propose developing a cellulosic yeast ethanologen that secretes a bactericidal lytic enzyme with specificity against contaminating lactic acid bacteria. Purified endolysins maintain their near-species specificity and can lyse both biofilm and senescent forms of Gram positive bacteria when exposed externally. The expression of these enzymes in yeast will be accomplished via a proprietary methodology that avoids inactivation due to aberrant glycosylation, a major pitfall of heterologous yeast expression systems.