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United States Department of Agriculture

Agricultural Research Service

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Research Project: Sirna-Mediated Knockdown/silence Post-Transcriptional Gene Expression of Chicken Ifn-Gamma

Location: Animal Biosciences and Biotechnology Laboratory

Project Number: 8042-32000-097-08
Project Type: Grant

Start Date: Jan 01, 2010
End Date: Dec 31, 2014

Objective:
To explore the possibility of initial stage of host gene manipulation by developing RNA interference technology using primary lymphocytes. We will use RNAi technology to modulate IFN-y response since IFN-y is a key immunoregulatory cytokine of macrophages for mucosal immune response.

Approach:
Peripheral blood monocytes will be cultured in vitro and treated with siRNA using recently developed RNA interference (RNAi) technology to knockdown/silence post-transcriptional gene expression of chicken IFN-y.

Last Modified: 10/23/2014
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