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United States Department of Agriculture

Agricultural Research Service

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Research Project: The Influence of Citrus Liminoids on Blood Concentration of Cholesterol and Inflammatory Responses in Humans

Location: Immunity and Disease Prevention Research Unit

Project Number: 5306-51530-017-05
Project Type: Trust

Start Date: Oct 01, 2010
End Date: Nov 30, 2012

Objective:
The pilot study will examine the metabolism, safety and efficacy of increased intake of citrus liminoids by human subjects. The main study will determine their health benefits, particularly the effects on serum lipids and markers of inflammation.

Approach:
In the pilot study 6 healthy subjects (18-65y) with LDL cholesterol greater than 130 mg/dL will be recruited and fed citrus liminoids. The metabolites of limonoids in the serum and urine samples will be determined by the use of mass spectrometry. NMR technology will be used to determine the number and sizes and of different lipoprotein fractions in plasma. Markers of inflammation in the serum and also their ex-vivo production after stimulation of blood cells with mitogens will be determined by ELISA and flowcytometry methods. The design, number of subjects, and the dose of liminoids to be used will depend upon the results obtained from the pilot study. Documents Trust with Cocoa Cola. Log 40626. Formerly 5306-51530-018-11T (12/01/2009).

Last Modified: 7/28/2014
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