Project Number: 5325-42000-048-04
Project Type: Reimbursable

Start Date: May 01, 2009
End Date: Apr 30, 2013

Objective:
1. To develop reagents for the sensitive detection of BoNTs in ELISA or ALISSA assay formats. 2. To optimize techniques for the recovery and detection of toxin in animal tissues and sera. 3. To determine the pharmacokinetics of botulinum neurotoxins in oral and parenteral mouse models of intoxication.

Approach:
Monoclonal antibodies directed against botulinum neurotoxins (BoNTs) serotypes B, E and F will be produced and used in sensitive in vitro assays to detect toxin in animal sera and tissues. Animals will be intoxicated and the half life and tissue distribution of the toxins determined. Documents Trust with UC Irvine. Log 36536. Formerly 5325-42000-043-04R (4/11)