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United States Department of Agriculture

Agricultural Research Service

Research Project: Enhance Variety Development of Scab Resistant Hard Winter Wheat Varieties in Nebraska Project Number: 0500-00053-002-93
Project Type: Grant

Start Date: May 03, 2009
End Date: May 02, 2014

Objective:
The objectives of this proposal are: 1. Breeding FHB-resistant hard winter wheat. 2. Growing the Quadri-state and NUWWSN nurseries along with the regional Germplasm Observation Nursery at 1-2 misted/inoculated locations. 3. Cooperating with the Regional Genotyping Center to rapidly develop lines with known and verified QTLs for FHB tolerance. 4. Cooperate with USDA-ARS scientists to sort kernels on hardiness, disease tolerance, and protein content (if heritable) to increase breeding efficiency so as to advance only those lines with acceptable market standards for scab testing. 5. Submit over 500 samples for DON testing.

Approach:
Obj. 1: We will make between 50 and 75 crosses having known FHB QTLs, mainly FHB1. The key parents used in this effort will be recently developed BC3F3 homozygous lines of Wesley. These lines will be crossed to our elite lines having native resistance. Obj. 2: We will grow the Quadri-state and NUWWSN nurseries and the RGON in one of our two misted inoculated locations to add important scab information for all winter wheat lines that have potential for release in scab affected areas nationally (NUSSWN), regionally in the primarily scab infected area (Quadri-state), as well as anywhere in the Great Plains (RGON). We will test the Nebraska State Variety Trial in the misted inoculated nursery to develop and provide information lines that are currently adapted or will be adapted in Nebraska to provide growers with FHB data onlines specifically marketed in Nebraska. Obj. 3: We will concentrate on the Wesley FHB1 lines and are increasing them in Arizona and Nebraska. The Harding lines were sent to South Dakota for their use. The genotyping center will screen segregating populations for FHB QTLs on chromosome 3B and 5A. Obj. 4: By sorting the population to remove soft kernels, we can greatly enrich the populations for hard wheat kernels, thus increasing the chance of finding adapted lines with the right market class and FHB resistance. Obj. 5: We will use bulk samples with replicated checks (to estimate error) from our mist nurseries of lines in the NUWWSN, Quadri-state, Nebraska State Variety Trial, Nebraska Interstate Nursery (our elite nursery), Triplicate (our advanced nursery), duplicate (our intermediate nursery), and those lines coming out of our FHB populations selections.

Last Modified: 4/21/2014
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