Project Number: 2050-21310-004-02
Start Date: Aug 19, 2009
End Date: Aug 18, 2014
Ingredients will be selected based upon nutrient and anti-nutrient profiles, and cost on a protein unit basis. Special emphasis will be placed upon barley and oat cultivars, but any ingredient that has favorable profiles will be considered. Some marine oils and proteins maybe evaluated at low inclusion levels to enhance feed palatability and the final product fatty acid profile. Practical feed formulations, will be produced using cooking extrusion (commercial conditions) for rapidly growing juvenile fish. Traditional evaluation procedures, including growth performance and nutrient retention, and cost per unit gain, will be used to evaluate the improved diet formulations. Additional evaluation procedures will be developed and applied to fish feeding trials as follows: (1) clinical assays of blood plasma for electrolyte levels, cholesterol, triglycerides, ASAT, ALAT, and routine plasma parameters; (2) whole blood analysis for hematocrit percentages; (3) gene expression activities for genes associated with protein synthesis and degredation, fatty acid elongation and desaturation, bone and/or cartilage metabolism and energy metabolism plus cellular redox status. Reproductive performance of rainbow trout fed diets containing plant-derived ingredients in place of fishmeal and fish oil (partial replacement or total replacement for selected growth periods) will be assessed by measuring fecundity, egg fertility, and egg hatchability, plus chemical analysis of eggs to determine effects of diet on nutritional content. Bone density and imaging may be explored for fish fed diets lacking fishmeal to determine if replacement mineral supplements in plant-based feeds support normal bone development and absence of skeletal deformities.