MOLECULAR GENETIC APPROACHES TO DEVELOP SCAB RESISTANCE
Project Number: 0500-00053-003-17
Start Date: May 17, 2009
End Date: May 16, 2014
Project #1: Characterize and Map Barley Genes that Respond to Fusarium graminearum Infection. Identify and characterize barley genes that respond to F. graminearum infection and trichothecene accumulation.
Project #2: Identify Barley Genes that Respond to Deoxynivalenol. Identify and characterize barley genes that respond to DON accumulation.
Project #3: Rapidly Identify and Test Scab Resistance Genes. Functionally characterize regulatory genes that respond to trichothecene accumulation and genes that encode enzymes that detoxify trichothecenes, and further test the function of NFX-1 and UDP-glucosyltransferase genes. There are two major objectives in the proposed work including: (1) rapidly test genes for FHB resistance/susceptibility; and (2) characterize the function of the NFX-1 and UDP-glucosyltransferase genes.
Project #1: (1) examine variation in genes that respond to Fusarium graminearum infection and trichothecene accumulation; (2) genetically map genes that respond to Fusarium graminearum infection and trichothecene accumulation; and (3) develop markers for barley breeding programs. In this proposal, we will sequence and map genes derived from our GeneChip experiments that respond to F. graminearum infection and trichothecene accumulation.
Project #2: (1) identify barley genes that respond to DON. We plan to use the Barley1 Genechip to examine transcript accumulation in barley spikes inoculated with DON and mock inoculated with water. We will analyze the data and identify the genes that respond to DON treatment. To refine the set of genes that respond to DON, we will compare the data obtained from the proposed GeneChip experiment with our previous GeneChip experiments.
Project #3: We will test a subset of these potential resistance genes in two functional assays including: (1) virus-induced gene silencing (VIGS); and (2) a yeast screen for UDP-glucosyltransferases that detoxify trichothecenes. We have also established a collaboration with Dr. Gerhard Adam to screen nine UDP-glucosyltransferases for the ability to detoxify trichothecenes.
We will develop and characterize transgenic wheat plants downregulating NFX-1 and upregulating the UDP-glucosyltransferase genes. These transgenic plants will likely result in wheat with enhanced resistance. Other genes that are identified in the VIGS or yeast assays will be transformed into wheat.