Start Date: Jul 13, 2009
End Date: May 23, 2013
1. Prepare cDNA libraries from various plant organs of the three legume species. Develop genomic libraries of the three species. 2. Use high throughput sequencing to evaluate transcriptome. 3. Sequence genes and prepare antibodies to the gene products. 4. Evaluate gene expression via RNA blots, immunoblots, enzyme assays, and in situ hybridization. 5. Characterize potential promoter elements for important genes related to the processes cited above. 6. Modify expression of relevant genes via overexpression, antisense, and/or RNAi approaches. BSL-1; Re-Certified June 12, 2007.