Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: INVESTIGATION OF SEEDLING YELLOWS CROSS PROTECTION BY MILD COMPONENTS OF DEKOPON CITRUS TRISTEZA VIRUS STRAIN

Location: Crop Diseases, Pests and Genetics

Project Number: 5302-22000-011-01
Project Type: Trust

Start Date: Nov 01, 2008
End Date: Oct 31, 2012

Objective:
1) Develop qPCR probes for the different Dekopon sub-isolates and quantify each in single and mixed infections to assess interference in accumulation/replication of each sub-isolate. 2) Examine P20, P23, and CP (suppressors of gene silencing) for each sub-isolate for genetic variation and associate with symptom expression. 3) Characterize siRNA profiles in mixed and single infected plants.

Approach:
Aphid transmission, reverse transcription (RT) polymerase chain reaction (PCR), real time PCR (qPCR), cloning, sequencing, western blots, northern blots, hybridizations. Documents Reimbursable with Citrus Research Board. Log 37119.

Last Modified: 4/17/2014
Footer Content Back to Top of Page