INVESTIGATION OF SEEDLING YELLOWS CROSS PROTECTION BY MILD COMPONENTS OF DEKOPON CITRUS TRISTEZA VIRUS STRAIN
Project Number: 5302-22000-011-01
Start Date: Nov 01, 2008
End Date: Oct 31, 2012
1) Develop qPCR probes for the different Dekopon sub-isolates and quantify each in single and mixed infections to assess interference in accumulation/replication of each sub-isolate.
2) Examine P20, P23, and CP (suppressors of gene silencing) for each sub-isolate for genetic variation and associate with symptom expression.
3) Characterize siRNA profiles in mixed and single infected plants.
Aphid transmission, reverse transcription (RT) polymerase chain reaction (PCR), real time PCR (qPCR), cloning, sequencing, western blots, northern blots, hybridizations. Documents Reimbursable with Citrus Research Board. Log 37119.