Location: Avian Disease and Oncology Laboratory
Project Number: 5050-31320-009-01
Start Date: Jan 01, 2009
End Date: Dec 31, 2012
1. Utilize chromatin immunoprecipitation (ChIP) using Meq or c-Jun antibodies and determine the DNA sequences via Solexa paired-end reads. 2. Isolate RNA from MSB-1 cells at time 0 and following MDV reactivation, and run on Affymetrix Chicken microarrays. 3. Compare by qRT-PCR the relative expression of key genes between MD tumors and CD4+ T cells. 4. Isolate RNA from line 6 (MD resistant) and line 7 (MD susceptible) CEF at various time before and after infection with either MDV or MDV lacking Meq, and run on Affymetrix Chicken microarrays.