Project Number: 6618-41430-005-03
Start Date: Jan 01, 2009
End Date: Dec 31, 2013
Initially we will be screening experimental compounds in the laboratory by in vitro testing with petri- dishes and pathogens of citrus to see if there is an indication of success with these compounds. This would involve using protocols already in place in addition to utilizing new methods for testing. Concurrently we will be testing packingline protocols already in place to see how much, if any, antimicrobial capabilities are already on the line. The compounds that are active against Xanthomonas citri subsp. citri (Xcc) will be tested on a pilot packingline at the Winter Haven facility. If these compounds are successful on this line, the protocol will be scaled up for trial in a commercial packinghouse. In the field we will be collecting fruit, twig and leaf lesions for the infection study. The lesions will be tested for biofilm development using fluorescent microscopy and changes in the microbial communities in the lesions will be evaluated by various isolation techniques and organisms identified by molecular protocols.