Project Number: 1245-32000-097-07
Project Type: Trust

Start Date: Apr 01, 2009
End Date: Mar 31, 2014

Objective:
The objective of this cooperative research project is to develop multiplex peptide nucleotide acid (PNA) chip and immune biomarkers associated with food-borne pathogens.

Approach:
NVRQS will develop multiplex PNA chip by identifying SNP of food-borne pathogens and validate PNA chip for diagnosis and detection of Clostridium, Salmonella and Campylobacter. ARS will develop disease challenge models to evaluate vaccines against poultry food-poisoning pathogen, develop and optimize real-time RT-PCR for detection of C. septicum and C. perfringens, develop ELISA for chicken cytokine detection to identify immune biomarkers associated with C. perfringens and C. septicum and evaluate potential vaccine candidates of Clostricium proteins.