Start Date: Aug 15, 2008
End Date: Aug 14, 2013
1. Fruit tissues representing different developmental stages will be collected for 12-16 cultivars, starting 30 days after full bloom till commercial maturity. 2. Apple fruits harvested at commercial maturity will be treated with1-MCP and fruit firmness changes of stored fruit will be assessed for at least 3 months. 3. Total RNAs will be isolated from above-mentioned fruit tissues using established procedure. 4. Gene-specific primers will be utilized to examine the expression patterns and dynamics on major ethylene biosynthesis enzyme encoding genes by quantitative real-time PCR methodology.