Start Date: Jun 01, 2008
End Date: May 31, 2012
1. Evaluation of mucosal adjuvants efficacy delivered through the ad5 platform, to induce mucosal immune responses to FMDV. Evaluate in vivo mucosal adjuvants alone or in combination with FMDV vaccine for induction of rapid protection in swine. Determine cross-neutralization and cross-protection provided by capsid-based vaccines engineered with chimeric VP1 G-H loops containing immunogenic or toleragenic epitopes. An epitope map will be created using anti-sera from murine bearing cross-reactive immune responses between FMDV types O and SAT3. Testing chimeric GH loop bearing hAd5 vectors in swine will be conducted to assess cross-neutralization. Challenge studies will be performed utilizing homo-typic and heterotypic virus. 2. Evaluate the role of non-structural proteins in CSFV virulence and protection against infection will be performed through; complete cloning of CSFV structural proteins into Baculovirus transfer vectors, completing the production of recombinant Baculovirus expressing parental CSFV structural proteins and of autonomous replication CSFV defective genomes, and by completing the immunogenicity studies in naïve swine with sera from infected swine. 3. Development and validation of the GCSPRI device will be done to use as rapid detection of FMDV and CSFV. Identify diagnostic reagents and develop host immune response characterization. Assay conditions and sensor chip configurations will be optimized to capture host leukocyte populations. In vivo virus detection will be tested through immune response by GCSPRI and by traditional bioassay.