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United States Department of Agriculture

Agricultural Research Service

Research Project: ANALYSIS OF FRUCTANS IN ORCHARDGRASS AND BERMUDAGRASS AS A FUNCTION OF DIURNAL AND SEASONAL VARIATION

Location: Forage-Animal Production Research

Project Number: 6440-21000-001-04
Project Type: Nonfunded Cooperative Agreement

Start Date: Apr 01, 2008
End Date: Mar 31, 2013

Objective:
Determine how fructan profiles in orchardgrass and bermudagrass forages are affected by the time of year in which they are grazed (early vs. late in the growing season), as well as by time of day.

Approach:
Orchardgrass and bermudagrass samples, collected in Virginia during the spring and late summer (mid-April to late August) will be analyzed for fructan content. Fructans will be extracted from the samples with boiling water and analyzed by anion-exchange high-performance liquid chromatography (HPLC) coupled to pulsed amperometric detection (PAD). Profiles will be compared to those of a sample harvested at the optimal growth stage to determine how seasonal and diurnal variations affect the relative changes in amounts and types of fructans in the forages.

Last Modified: 9/1/2014
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