BIOLOGICAL AND GENETIC ANALYSIS OF POTATO VIRUS Y ISOLATES AFFECTING THE U. S. POTATO CROP
Project Number: 1907-22000-021-05
Specific Cooperative Agreement
Start Date: Jul 01, 2008
End Date: Jun 30, 2013
1. Sequence whole genomes for at least 3 PVY-N:O strains that do not cause tobacco vein necrosis, and analyze their sequences against at least 3 other PVY-N:O strains that do.
2. Sequence whole genomes for at least 3 PVY isolates that cause tuber necrosis in potato and analyze their sequences against at least 3 other PVY strains that do not.
3. Express PVYO and O5 capsid proteins in bacteria and correlate the monoclonal 1F5 reactivity with the point mutation in the PVY-O capsid protein.
Potato virus Y isolates collected by State Potato Seed Certification agencies and partially characterized by either State laboratories or the ARS laboratory at Ithaca, NY will be sent to the University of Idaho. Virus genomes will be amplified using a RT-PCR protocol and the products sequenced. Sequence information will be catalogued in a bioinformatics database that will be made available to all collaborators on the project. Virus protein fragments will be expressed in bacteria and used to characterize epitopes that recognize monoclonal antibodies.