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United States Department of Agriculture

Agricultural Research Service

Research Project: Genomics Based Discovery of Novel Traits in Beneficial Plant-associated Pseudomonas Species Project Number: 5358-12220-003-11
Project Type: Grant

Start Date: Apr 09, 2008
End Date: Dec 31, 2010

Objective:
1. Generate draft genomic sequences for seven plant-associated strains of Pseudomonas spp. that suppress plant disease. 2. Generate assemblies of the genomes. 3. Perform automatic annotation of the genomes. 4. Provide an instructor and materials for a workshop for 14 collaborators.

Approach:
Prepare random genomic shotgun libraries and sequence both ends of randomly selected clones. Assemble genome from shotgun libraries and sequencing. For initial identification of protein coding sequences, the Glimmer algorithm will be used. All regions of a genome without Glimmer predictions will then be re-evaluated using BLASTP to search against a database of non-redundant proteins (nraa) maintained at JCVI.

Last Modified: 10/23/2014
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