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United States Department of Agriculture

Agricultural Research Service

Research Project: ASSAY DEVELOPMENT FOR DETECTION OF PATHOGENIC E. COLI IN ENVIRONMENTAL SAMPLES

Location: Environmental Microbial and Food Safety Laboratory

Project Number: 1245-12630-008-12
Project Type: Nonfunded Cooperative Agreement

Start Date: Mar 06, 2008
End Date: Mar 05, 2013

Objective:
The objective of this cooperative research project is to develop an assay for the detection of pathogenic E. coli in water, soil, manure and food matrices using an integrated immuno-PCR biosensor.

Approach:
Pathogenic E. coli will be captured and fluorescently labeled with specific monoclonal antibodies. The E. coli cell-antibody complex will subsequently be detected using an immunoassay biosensor followed by PCR characterization.

Last Modified: 8/1/2014
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