ASSAY DEVELOPMENT FOR DETECTION OF PATHOGENIC E. COLI IN ENVIRONMENTAL SAMPLES
Environmental Microbial and Food Safety Laboratory
Project Number: 1245-12630-008-12
Nonfunded Cooperative Agreement
Start Date: Mar 06, 2008
End Date: Mar 05, 2013
The objective of this cooperative research project is to develop an assay for the detection of pathogenic E. coli in water, soil, manure and food matrices using an integrated immuno-PCR biosensor.
Pathogenic E. coli will be captured and fluorescently labeled with specific monoclonal antibodies. The E. coli cell-antibody complex will subsequently be detected using an immunoassay biosensor followed by PCR characterization.