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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFYING DNA MARKERS LINKED TO THE FUSARIUM WILT (RACE 1) RESISTANCE GENE IN WATERMELON

Location: Vegetable Research

Project Number: 6659-21000-017-01
Project Type: Nonfunded Cooperative Agreement

Start Date: Nov 28, 2007
End Date: Nov 28, 2012

Objective:
To map and develop molecular markers linked to the fusarium wilt resistance (race 1) gene in watermelon.

Approach:
The cooperator has developed parental lines and BC1 and F2 populations segregating for FW (race 1) resistance. Previous experiments conducted by the cooperator with these populations showed that the FW (race 1) resistance is controlled by a single dominant gene. The cooperator grew 200 F2 plants and collected leaf samples for DNA analysis from each of these F2 plants. The cooperator is self-pollinating each of the F2 plants in order to produce F3 families. These F3 families will be used by the cooperator for progeny evaluation and identifying the FW (race 1) resistant and susceptible F2 plants. The cooperator will provide ARS the F3 progeny evaluation data and leaf samples of the F2 plants (and their resistant and susceptible parental lines). The ARS scientists will isolate DNA from each of the parental lines and F2 leaf samples, and conduct genetic mapping analysis to identify markers linked to the FW-resistance gene.

Last Modified: 9/2/2014
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