Location: Molecular Plant Pathology
Project Number: 1245-22000-282-02
Start Date: Aug 23, 2007
End Date: Jul 31, 2012
Analyze spiroplasma and phytoplasma genome sequences using bioinformatics tools. Establish clone libraries of selected spiroplasma and phytoplasma genes. Engineer gene constructs for expression of individual pathogen genes. Functional test of cloned spiroplasma and phytoplasma genes will be carried out in experimental host plant Nicotiana benthamiana using a Potato virus X-based vector. Inoculated plants will be examined for abnormal phenotypes caused by expression of the introduced pathogen genes. Once such abnormal phenotypes are observed, further studies will be performed on two fronts (i) mutational studies on the pathogen genes that elicited the phenotypes, and (ii) dissection of signal transduction events that lead to the host responses.