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United States Department of Agriculture

Agricultural Research Service

Research Project: CONFIRMATION OF CLAVIBACTER MICHIGANENSIS SUBSP. SEPEDONICUS INFECTIONS USING A MULTIPLEX REAL-TIME PCR ASSAY FOR RING ROT DIAGNOSIS (NDSU)

Location: Vegetable and Forage Crops Production Research

Project Number: 5354-21220-010-07
Project Type: Specific Cooperative Agreement

Start Date: Sep 21, 2007
End Date: Jul 31, 2012

Objective:
Confirmation of Clavibacter michiganensis subsp. sepedonicus infections using a multiplex real-time PCR assay for ring rot diagnosis and potato seed lot screening.

Approach:
Real-time PCR primers and fluorescently labeled Taqman probes were previously developed for detection of three unique areas of the bacterial genome of C. michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot (BRR) of potato. These genomic regions code for cellulose genes (CelA and CelB) that are primary virulence determinants for Cms. These reagents will be used for additional screening of bacterial potato endophytes by a multiplex real-time PCR to verify specificity and reliability of the procedure for Cms detection and identification. Documets SCA with N. Dakota State University. Formerly 5354-21220-002-20S (5/08).

Last Modified: 4/19/2014
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