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United States Department of Agriculture

Agricultural Research Service

Research Project: APPLICATION OF NEW DIAGNOSTIC REAGENTS FOR IDENTIFICATION OF DIVERSE STRAINS OF POTATO VIRUS Y

Location: Vegetable and Forage Crops Production Research

Project Number: 5354-21220-010-06
Project Type: Specific Cooperative Agreement

Start Date: Sep 18, 2007
End Date: Jul 31, 2012

Objective:
Develop new monoclonal antibodies that can be used for rapid detection and identification of Potato virus Y (PVY) strains that can be utilized on a large scale by certification agencies, researchers, and diagnostic facilities.

Approach:
Nucleoproteins of diverse strains of PVY will be purified using standard methods. These will be used to immunize mice for production of monoclonal antibodies (MAbs) by hybridoma technology. The MAbs will be tested for virus strain specificity using a large panel of characterized PVY isolates available to the cooperator. Documents SCA with University of Idaho. Formerly 5354-21220-002-19S (5/08).

Last Modified: 4/20/2014
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