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United States Department of Agriculture

Agricultural Research Service

Research Project: CYTOGENETIC ANALYSIS OF INTERSPECIFIC COTTON POLYPLOIDS AND DEVELOPMENT OF TETRAPLOID RANDOM-MATED BREEDING POPULATIONS

Location: Crop Genetics Research Unit

Project Number: 6066-22000-074-03
Project Type: Nonfunded Cooperative Agreement

Start Date: Dec 15, 2006
End Date: Sep 30, 2011

Objective:
The objective of this cooperative research project is to identify interspecific cotton progenies that have been converted from sterile triploids into fertile hexaploids, and to determine which chemical compounds and concentrations are most effective at producing such conversions. In addition, interspecific tetraploid breeding populations will be developed.

Approach:
Triploid progenies of Gossypium hirsutum/G. arboreum will be treated with amiprofosmethyl, colchicine, oryzalin, or trifluralin at four concentrations each. Cuttings of each triploid cross will be made and axillary buds treated with the dissolved compounds mixed in a lanolin paste. Controls will include no paste and water-lanolin. The number of self-fertile cuttings per treatment will be recorded. Chromosome counts will be conducted to confirm the ploidy of fertile plants. When tetraploid progeny of the interspecific hybrids are produced, random mated breeding populations will be developed to break up linkage blocks.

Last Modified: 10/23/2014
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