Location: Grain, Forage & Bioenergy Research
Project Number: 5440-21000-031-12
Start Date: Aug 01, 2014
End Date: Jul 31, 2019
To increase lignin synthesis wheat, we will introduce three constitutive expression cassettes under control of the 35S promoter. We will constitutively express the genes encoding 3 enzymes in the monolignol pathway: caffeoyl CoA O-methyltransferase, coumarate 3-hydroxylase, 4-coumarate-CoA ligase, in Chinese spring wheat CB037. Agrobacterium-mediated transformation will be used coupled to npt II selectable marker. For each transgenic cassette a minimum of 10 independent events will be generated. Transgenic lines will be evaluated for resistance to stem rust and sawflies in greenhouse environments. Successful transformation events will be transferred to winter wheats via traditional breeding approaches.