Location: Sugarcane Research Unit
Project Number: 6052-21000-015-15
Start Date: Jul 01, 2014
End Date: Jun 30, 2019
Currently, there are 2,408 Saccharum accessions listed in the Germplasm Resources Information Network (GRIN) database representing 21 different species. Leaf samples will be collected, and DNA will be extracted from all these accessions using a FastPrep tissue homogenizer and a mini CTAB procedure. The accessions will be genotyped using microsatellite and ribosomal DNA markers. A set of highly polymorphic microsatellite markers has already been identified at the LSU AgCenter in the Lead PD’s lab from the ongoing research with commercial varieties and Louisiana parental clones. Analysis of the marker data will be used to estimate genetic (dis)similarity among the clones currently present in the collection. In addition, duplicates in the existing germplasm will be identified. A sugarcane core collection will be identified based on this analysis.