Location: Sunflower Research
Project Number: 3060-21220-028-26
Start Date: Jun 01, 2014
End Date: Sep 30, 2016
For objective 1, over 70 advanced breeding lines derived from multiple sources of resistance to Sclerotinia will be evaluated at seven locations in Michigan for yield and agronomic traits. The lines will also be evaluated for resistance to SSR in a Sclerotinia disease nursery and in the greenhouse with the drop-mycelium method developed by us. About 15% of the lines will be selected based on their resistance to SSR, yield, and other agronomic traits. The selected lines will be re-evaluated in our disease nursery for resistance to SSR and yield under disease pressure. Five to 10 lines will be selected and tested in the Uniform Soybean Tests – Northern Region (19 locations in 10 northern US states and 1 Canadian province), for yield and other agronomic traits. The best lines will be released to the public. For objective 2, a diverse set of early maturing (maturity groups I, II and III) 280 soybean plant introductions selected based on 52,041 SNP marker data will be evaluated for resistance to SSR in a naturally infected Sclerotinia disease nursery and in the greenhouse with the drop-mycelium method. For objective 3, over 500 breeding lines developed by crossing SSR resistant parents with SSR susceptible parents will be evaluated for resistance to SSR in the disease nursery and in the greenhouse. These lines will also be genotyped with SNP markers that were found associated with SSR resistance in our genome wide association studies. The SNP marker data and the disease resistance data will be analyzed together to validate the association of the SNP markers with resistance to SSR.