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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Genetics Approaches to Developing Scab Resistance Project Number: 0500-00053-003-81
Project Type: Grant

Start Date: May 17, 2014
End Date: May 16, 2015

Objective:
Project 1 - Molecular Genetics Approaches to Developing Scab Resistant Barley: (1) characterize transgenic barley overexpressing HvUGT13248; and (2) fine map and characterize the chromosome 6H bin 7 FHB resistance QTL. Project 2 - Rapidly Identifying Scab Resistance Genes and Developing Scab Resistant Wheat: (1) develop elite wheat cultivars with FHB resistance; (2) characterize the accumulation of trichothecenes and trichothecene-glucoside conjugates in transgenic wheat carrying HvUGT13248; and (3) test potential trichothecene resistance genes.

Approach:
Project 1: We will conduct two backcrosses of the transgenic plants into the Rasmusson genetic background and during the introgression process we will select for expression of the transgene. Homozygous lines will be screened for FHB resistance in the field. Obtain genetic stocks segregating for the chromosome 6H bin 7 region and select recombinants from ~5,000 individuals using flanking markers. Screen the recombinants for FHB severity in the field and assess protein content in the mature grain. Project 2: Introgress the HvUGT13248 transgene into two elite cultivars: Linkert and Rollag. Characterize the accumulation of trichothecenes and trichothecene-glucoside conjugates in transgenic wheat carrying HvUGT13248. All genes that exhibit resistance will be transformed into wheat and tested in the greenhouse and field.

Last Modified: 10/21/2014
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