Location: Tick and Biting Fly Research
Project Number: 6205-32000-036-46
Start Date: May 01, 2014
End Date: Apr 30, 2018
1. Illumina RNA-Seq approach will be used for deep sequencing the transcriptome of the horn fly. This approach is estimated to acquire approx. 95% of the transcribed genes of the organism. Selected transcriptome analysis of dissected organs or tissues could be targeted by this approach, with the midgut a likely tissue of interest. Bioinformatic analysis will also be required to assemble and annotate this data. 2. Pacific Biosciences Third Generation sequencing technology will be used for library synthesis and SMRT cell protocol optimized for long read sequencing. Specialized bioinformatic analysis will be required for interpretation, assembly, and annotation of this dataset.