Location: Screwworm Research
Project Number: 3094-32000-035-05
Start Date: May 01, 2014
End Date: Dec 31, 2014
The project has advanced to the stage where evaluations of existing female conditional lethal vectors have been initiated in the Screwworm Research Unit's facility at Pacora, Panama: this work will continue. We will also develop a second vector system as a contingency in the event the current vectors do not meet the Eradication Program's needs. We will: 1. Develop tetracycline repressible female lethal conditional lethal cloning vector based on improved piggybac transposable element/helper system, C. hominivorax transformer sex-specific splicing intron, tTAV coding region, and tetO operon/promoter. 2. Evaluate the new vector through transformation experiments in the secondary screwworm Cochliomyia macellaria before transferring to C. hominivorax (in Panama).