Cryotherapy of Horticultural Crops
Plant Germplasm Preservation Research Unit
Project Number: 5402-21000-014-09
Specific Cooperative Agreement
Start Date: May 01, 2014
End Date: Apr 30, 2019
Develop cryotherapy technologies to eradicate diseases from Horticultural crops, primarily Citrus. 1) Develop cryopreservation technologies. 2) Use these technologies to screen diseased plant materials and determine if diseases have been eliminated. 3) Optimize techniques, as necessary, for improved regrowth of plants after liquid nitrogen treatments.
Cryopreservation techniques will involve the use of tissue culture, vitrification cryopreservation methods, microscopy, as well as molecular tools to determine the presence or absence of pathogens. Experiments will be performed to optimize survival of plant tissues and eradication of bacteria, viruses, and/or viroids after liquid nitrogen treatment.