Location: Plant, Soil and Nutrition Research
Project Number: 8062-52000-001-00
Start Date: Jan 29, 2014
End Date: Jan 28, 2019
This project has two major objectives. The first is to develop lines of staple food crops with improved bioavailability and content of iron (Fe). To do so we will employ our established screening tools of an iron bioavailability bioassay and an animal model. In conjunction with these tools, we will make use of mass spectroscopy and marker assisted molecular breeding techniques. With these approaches, we will be able to identify and utilize regions of the plant genome that harbor genes associated with the enhanced food crop nutritional quality for Fe. To further complement the above approaches, we will continue our work on the identification of agricultural and food processing practices that enhance the bioavailability and content of Fe and Zn in staple food crops. The second major objective of this project is to develop a method to measure and evaluate the bioavailability of functional phytochemicals from plant foods. To do so, we will assess the use of a surgical model utilizing the unique intestinal anatomy of the broiler chicken to measure absorption of compounds from the duodenal segment of the intestine. This approach uses an established surgical preparation involving anesthetized animals. Our initial efforts with this model will focus on compounds that are common to many staple food crops, fruits and vegetables. These phytochemicals will include the flavonoids epicatechin, quercitin, caffeic acid, chlorogenic acid and kaempferol. Enhancing our knowledge of bioavailability of health promoting phytochemicals will significantly improve efforts to develop more nutritious plant foods.