Location: Foreign Animal Disease Research
Project Number: 8064-32000-057-64
Start Date: Jan 31, 2014
End Date: Feb 01, 2017
To improve the potency and efficacy of current Ad5-FMD vaccine candidates, we will examine the use of alternative routes of inoculation and novel adjuvants. We will study the efficacy of Ad5-FMD vaccines alone or in combination with adjuvants such as toll like receptor agonists or mucosal specific enterotoxin adjuvants administered parenterally by subcutaneous inoculation or transdermal administration using a needle free device. Since FMDV is an antigenically variable virus and consists of 7 serotypes and multiple subtypes, protection against this disease requires production of multiple vaccines. One mechanism of potentially broadening or refocusing the immune response is to alter variable immunodominant epitopes on the pathogen potentially allowing for a more robust response to more conserved neutralizing epitopes. We will substitute various portions of the immunodominant G-H loop of VP1 in the Ad5-FMD vector with foreign epitopes and examine the ability of the redesigned vectors to induce cross neutralization of heterologous viruses within the same serotype. We will continue to develop improved Ad5-FMD vectors and as information is obtained from the above approaches the best candidates will be tested with the improved vector platforms.