Start Date: Dec 01, 2013
End Date: Jul 31, 2016
Combining our expertise in cytogenetics, molecular genetics, and bioinformatics, we first address the question of whether MD vaccines or genetic resistance status of the chicken alters the spectrum of MDV integration sites in the chicken genome, which we have recently shown to occur early and is part of the natural life-cycle of both pathogenic and avirulent MDV strains. We predict that there will be a host genotype by MD vaccine serotype interaction, which will provide valuable information on how the mechanisms of MD vaccines and MD genetic resistance interact. Second, we address whether genomic alterations are necessary for MDV-induced transformation. Using custom SNP chips and next generation sequencing, we will determine if MD tumors exhibit loss of heterozygosity (LOH), copy number variation (CNV), or other alterations in the chicken genome. We will also determine whether MDV integrates directly into the telomeres or only near them in specific regions.