Location: Sunflower Research
Project Number: 3060-21220-028-24
Start Date: Jul 01, 2013
End Date: Sep 30, 2016
Our approach to accomplish the overall project goals is being carried out over the course of three years. Thus far in the first two years we have completed the fine-scale genetic mapping of the hss1 mutation, verified the function of the HSS1 gene in Arabidopsis and developed plants that over-express the HSS1 and ODC2 genes. The goals for year three are to assay disease resistance in HSS1/ODC2 Arabidopsis transgenic lines, to test the function of BnHSS1, and to engineer resistance in canola using HSS1 and ODC2. This project has direct relevance to the goals and priority research needs of the Sclerotinia Initiative. The proposed studies will make significant contributions towards understanding the genetics of host resistance and directly improving crop germplasm for Sclerotinia resistance. Although the first and second year objectives have been concerned primarily with the non-crop plant A. thaliana; this has been integral to our success in mapping the HSS1 locus and our overall project goal of producing resistant canola lines. We are beginning now to evaluate HSS1/ODC2-expressing lines for disease resistance phenotypes. In the current funding request for year three, we propose to identify the functional BnHSS1 gene and verify its activity by complementation of the Arabidopsis hss1 mutant. Meanwhile, we will generate B. napus lines overexpressing HSS1 and ODC2. We have chosen to initially focus on canola as it is a crucifer with a close relationship to A. thaliana and also due to our experience with 3 transgenic manipulation of canola. In the long term, we anticipate that the results produced here will be applicable to increasing resistance in other susceptible crops including soybean, dry bean, sunflower and pulse crops.