Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Evaluation of Bovine Coronavirus Field Strains for Use As Vaccine Or Challenge Viruses Project Number: 3625-32000-106-18
Project Type: Trust

Start Date: May 30, 2013
End Date: May 30, 2016

Objective:
Evaluate bovine coronavirus (BoCV) field isolates using in vitro assays based on infection of cultured macrophages.

Approach:
Develop in vitro assay using cultured macrophages to compare BoCV strains. Macrophages,isolated from blood of non infected donor animals (bovine), will be cultured in the presence of different BoCV strains. Viral titer will be monitored by VI and PCR and pro-inflammatory cytokines will be monitored using real-time PCR a multiplex ELISA assay.

Last Modified: 7/22/2014
Footer Content Back to Top of Page