Start Date: Apr 01, 2013
End Date: Mar 29, 2015
A Bacillus-vectored vaccine expressing epitopes from the AI HA, NP and M2e proteins will be generated by PGT (Pacific GeneTech Limited, Hong Kong). Groups of 8-10, one-day-old commercial chickens will be injected with inactivated bacillus-AI vaccine. After 21 days, the birds will be transported to SEPRL for challenge with LPAI H7N2 under BSL3Ag conditions. Protective immunity will be evaluated by morbidity /mortality to challenge, with oral and cloacal swabs taken to determine viral shedding. Humoral immunity will be evaluated by ELISA and/or HI.