Location: Peanut Research
Project Number: 6604-21000-004-00
Start Date: Jul 30, 2013
End Date: Jul 29, 2018
The causal agents of leaf spot will be isolated from symptomatic leaves to determine their genetic diversity. We will develop molecular markers and fingerprint the isolates. We will also develop molecular markers for peanut plants. Multiple peanut lines will be grown in multiple locations, phenotyped for leaf spot resistance, and fingerprinted with molecular markers to find association between markers and disease resistance. For the use of RNA interference (RNAi) in the control of aflatoxin, multiple approaches will be used, including making multiple constructs that target 2-5 genes in the pathway, genetic transformation of Aspergillus spp., transformation of Arabidopsis to characterize the effectiveness of the system, genetically transform peanut plants with the most promising constructs and finally test for accumulation in seeds after challenging them with aflatoxigenic strains of the pathogen. For drought tolerance, multiple peanut genotypes that have shown different response to drought will be grown in multiple locations, under drought and irrigation conditions. Genes potentially regulated by drought will be identified from transcriptome sequencing, also genes potentially regulated by epigenetics (i.e., small RNAs, methylation) will be identified and later verified the expression profiling. Both, genes and molecular markers identified in the various areas, will be incorporated into the breeding program.