Genotypic Evaluation and Marker Selection of Lolium and Festuca Germplasm
Forage and Livestock Production Unit
2013 Annual Report
1a.Objectives (from AD-416):
Screen a series plant germplasm for the presence/absence of a series of Lolium SSR markers. Germplasm that will be evaluated in the project are IL1, IL2, IL x Festuca hybrids and Festuca dihaploid recoveries.
1b.Approach (from AD-416):
Lolium multiflorum and L. perenne SSR markers will be evaluated across a series of Lolium, Festuca and Lolium x Festuca hybrids; as well as their dihaploid offspring. Initial analysis will follow standard PCR methodology and evaluations will be performed on horizontal based agarose gel electorophoresis. Exceptional markers then will be re-evaluated on a capillary electrophoresis apparatus to determine their validity and obtain a more accurate base-pair size reference. Germplasm to be utilized in the study will be provided by the USDA-ARS. Seed and or plant material will be transferred to cooperators at Cameron University for PCR and marker evaluation
There has been no progress in the cooperation with Cameron University. There is nothing to report.