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United States Department of Agriculture

Agricultural Research Service

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Research Project: Application of Molecular Marker-Assisted Selection to Rice Improvement (Continuing Project)

Location: Crops Pathology and Genetics Research

Project Number: 5306-21000-021-04
Project Type: Trust

Start Date: Jan 01, 2013
End Date: Dec 31, 2013

Objective:
Research will be conducted on the development and application of methods and protocols for genotyping of rice using next-generation DNA sequencing and on the development and characterization of genetic mapping and mutant populations of rice. Specific objectives include: 1) next-generation genotyping of rice germplasm; 2) sequencing of expressed rice genes from California varieties; 3) development and screening of rice populations for genetic analysis of agriculturally important traits.

Approach:
Single nucleotide polymorphisms (SNPs) will be identified by sequencing reduce representation libraries of selected rice germplasm in conjunction with computational genomics. SNP markers identified in this manner will be used to map traits of interest where possible. Exome sequencing will be employed to sequence protein coding regions of the genomes with emphasis on proteins implicated in yield and tolerance to abiotic stress. Development of genetic mapping and mutant populations will consist of generation advance through single-seed descent. Screening populations for agronomic traits of interest will be performed in consultation and conjunction with Rice Experiment Station breeders.

Last Modified: 7/23/2014
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