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United States Department of Agriculture

Agricultural Research Service

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Research Project: Large Scale Identification of Economically Important Wild Soybean Diversity and Transfer to Applied Breeding

Location: Soybean Genomics and Improvement

Project Number: 8042-21000-275-42
Project Type: Reimbursable

Start Date: May 01, 2013
End Date: Dec 31, 2013

Objective:
The collaborators have created a number of large populations from crossing high yielding elite soybean cultivars with a diverse set of wild soybeans from the USDA, ARS, Soybean Germplasm Collection. The Collaborators are growing large numbers of the resulting progeny in the field and selecting progeny with the most desirable agronomic, pest resistance and seed quality traits. The ultimate goal is 1) to identify genes or genome regions from the wild soybean that can be introgressed into cultivated soybean and that will result in enhanced soybean productivity, and 2) to identify DNA markers that can be used in soybean breeding to identify breeding lines that carry the genes or genome regions that result in enhanced productivity.

Approach:
The collaborator at the USDA, Raleigh, North Carolina; the USDA, Urbana, Illinois; and the University of Missouri have made crosses between a diverse set of 35 wild soybeans from China, Japan, Russia and Korea that represents much of the genetic diversity that is present in wild soybeans in the USDA Soybean Germplasm Collection. Preliminary analysis indicates that each wild soybean included in this project will differ from elite soybean varieties by 400 to 800 single nucleotide polymorphism (SNP) DNA markers out of a total of 1563 SNP DNA markers. The goal is to transfer all the different SNP marker alleles identified in each wild soybean accession to a set of 30 or less adapted breeding lines. This set of 30 lines will constitute a large reservoir of wild soybean genes that will be readily available for soybean variety improvement. Any new breeding lines that yield at least 90% of soybean variety checks will be released immediately. Approximately 200 agronomically adapted breeding lines will be retained from each population for identification of putative yield genes, development of near isogenic lines for verification of yield genes, and further breeding work. In order to properly identify the genes or regions of the genome that carry such genes whole genome DNA sequence analysis will be performed on each of the 35 wild soybean parents as well as the elite soybean varieties that were used by the collaborators as parents in the cultivated by wild soybean crosses. The DNA sequence analysis will be performed at the USDA Beltsville, MD. The bioinformatic analysis of the resulting sequence data will also be conducted at the USDA, Beltsville. In subsequent years, after completion of selection and yield testing of the progeny from the cultivated x wild soybean crosses the whole genome sequence of a small set of the highest yielding progeny from each cross will also be determined. Comparisons of the whole genome sequence of the wild soybean, the elite soybean parents and the highest yielding progeny will be used to identify genes and/or genome regions from the wild soybean that enhance productivity of the soybean progeny and that are currently not present in the elite soybean cultivars. These DNA sequence comparisons will also be used to identify DNA markers that can be used to identify new soybean breeding lines that contain the desired genes and/or genome regions from the wild soybean that increase productivity.

Last Modified: 12/24/2014
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