Project Number: 3625-21000-058-00
Start Date: Apr 14, 2013
End Date: Apr 13, 2018
Obj 1: Regeneration/management focus on species or accessions is subject to demand for plant germplasm/genetic resources (PGR), external pressures, and prioritized using many factors. Controlled pollination techniques will be used for regeneration in field and greenhouse plantings, meeting pollination and biological requirements. Seeds/plantings will be monitored for disease and assayed for pathogen identity. Phytosanitary precautions will be implemented at several points to preclude seed-borne pathogens. Seed lots will be backed up at the NCGRP and the Svalbard Global Seed Vault. Periodic germinations will assess collection quality and health to assure their security, and new protocols developed. Quality PGR and information will be distributed to support research objectives based on criteria, applicable phytosanitary, importation or IP restrictions, threatened/endangered status, noxious weed status, etc., which may govern distribution or use. Obj 2: In collaboration with the DBMU, cooperators and stakeholders, develop/enhance/deploy GRIN-Global (GG) to provide superior user interfaces and expanded capacities to manage PGR information. We will enhance/optimize the Curator Tool, Search Tool, Order Wizard, and Accession Wizard; develop wizards to manage crop traits, inventory, and images; oversee system architecture; enhance system design to simplify installation/maintenance. Collaborate with model organism and other information system providers to simplify and streamline data delivery by supporting more data formats delivered by the middle tier, thus facilitating information access/retrieval with other providers to join GG System information with theirs in order to support complex analytical needs. Obj 3: Geospatial, genetic, phenotypic and other information resources for key cultivated crops and crop wild relatives (CWR) will be collected; analyses will help determine gaps in collection holdings and/or information, establish/analyze accession relationships, and refine management strategies. Climatic site analogs for known occurrence of taxa will be used to determine support for occurrence of unique crops and CWR in areas representing 1°, 2°, or 3° gene pools. Collection plans to acquire key diverse PGR will be developed using research, stakeholder input, and assistance of PEO personnel. Collection opportunities, climatic and social/political considerations impact priorities. Results will support curatorial decision-making and better representation of ecogeographic and genetic diversity. Obj 4: Collaborative genetic/genomic characterization and phenotypic evaluations for priority traits, i.e. host-plant resistance to biotic and abiotic stresses, will be conducted. During the regeneration process, phenotypic descriptors will be captured using accepted data standards. Molecular detection methods for differentiating Pantoea stewartii from related species will be refined to support development and implementation of accurate detection procedures. Data will be incorporated into GRIN/GG or other databases. Trait ontologies will be aligned with GRIN/GG descriptors to facilitate interoperability and for the benefit of research communities.