Location: Crop Diseases, Pests and Genetics
Project Number: 5302-22000-011-06
Start Date: Jan 01, 2013
End Date: Dec 31, 2013
Use and modify commercial kits which capture nucleic acid on magnetic beads during robotic extraction to yield high quality total nucleic acid; utilize existing literature and validate or improve real-time PCR detection of specific RNA and DNA targets; develop a custom micro-fluidic card with multiple pathogen target signatures (primer sets) for amplicon reaction with TaqMan® probes; use a block specifically designed to conduct qPCR with 384-well microfluidic cards.