1a.Objectives (from AD-416):
The objective of this work is to develop Easter lilies that are resistant to nematodes.
1b.Approach (from AD-416):
Transgenic lily lines developed previously will be tested in vitro for nematode resistance, and new nematode resistance genes will be isolated. Promising lines will be sent to the Easter Lily Foundation for further evaluation.
The cooperator has sent ARS lily bulbs for in vitro propagation and a soil sample from the field where the lilies are grown for the Easter Lily Foundation. Pratylenchus penetrans, the root lesion nematode, was confirmed to be in the soil sample. ARS has isolated a part of the unc and pat genes from P. penetrans for future use in genetic engineering of lilies for nematode resistance. ARS is challenging transgenic lilies that contain the cystatin gene with P. penetrans to determine if cystatin confers resistance to the nematode.