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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Immunodiagnostics to Detect Prions and Other Important Animal Pathogens

Location: Produce Safety and Microbiology Research

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Detecting and discriminating among Shiga toxins - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
Using small molecule reagents to help distinguish among prion structural models - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A. 2016. Using small molecule reagents to help distinguish among prion structural models. Prion 10(Supplement 1). p. S37-S38.
Identification of new molecular alterations in Fatal Familial Insomnia - (Peer Reviewed Journal)
Llorens, F., Thune, K., Schmitz, M., Cramm, M., Tahir, W., Gotzmann, N., Zerr, I., Silva, C.J., Frau-Mendez, M.A., Ansoleaga, B., Berjawi, S., Carmona, M., Ferrar, I., Fernandez, I., Zarranz, J. 2016. Identification of new molecular alterations in Fatal Familial Insomnia. Human Molecular Genetics. pii: ddw108.
Covalent surface modification of prions: a mass spectrometry-based means of detecting distinctive structural features of prion strains     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.C. 2016. Covalent Surface Modification of Prions: A Mass Spectrometry-Based Means of Detecting Distinctive Structural Features of Prion Strains. Biochemistry. 55:894-902.
Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera - (Peer Reviewed Journal)
Babrak, L.M., Lin, A.V., Stanker, L.H., McGarvey, J.A., Hnasko, R.M. 2016. Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera. Toxins. 8(1):13.
Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
Monoclonal antibody technologies and rapid detection assays - (Proceedings)
Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A. 2015. Monoclonal antibody technologies and rapid detection assays.September 23-25, 2015 Carlsbad, CA: p. 1-44.
ELISA: Methods and Protocols - (Book / Chapter)
Hnasko, R.M. 2015. ELISA: Methods and Protocols. Methods in Molecular Biology. Volume 1318. New York: Humana Press. 435 p. doi: 10.1007/978-1-4939-2742-5.
The biochemical properties of antibodies and their fragments - (Book / Chapter)
Hnasko, R.M. 2015. The biochemical properties of antibodies and their fragments. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 1-14. doi: 10.1007/978-1-4939-2742-5.
Hybridoma technology - (Book / Chapter)
Hnasko, R.M., Stanker, L.H. 2015. Hybridoma technology. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 15-28. doi: 10.1007/978-1-4939-2742-5.
Bioconjugation of antibodies to horseradish peroxidase (hrp) - (Book / Chapter)
Hnasko, R.M. 2015. Bioconjugation of antibodies to horseradish peroxidase (hrp).In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 43-50. doi: 10.1007/978-1-4939-2742-5.
Affinity purification of antibodies - (Book / Chapter)
Hnasko, R.M., McGarvey, J.A. 2015. Affinity purification of antibodies. Methods in Molecular Biology. DOI: 10.1007/978-1-4939-2742-5.
The western blot - (Book / Chapter)
Hnasko, R.M., Hnasko, T.S. 2015. The western blot. In: Hnasko, Robert, editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY:Humana Press. p. 87-96. doi: 10.1007/978-1-4939-2742-5.
Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices - (Peer Reviewed Journal)
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Hnasko, R.M. 2015. Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7:1163-1173.
The chemistry of prions: small molecules, protein conformers and mass spectrometry     Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2015. The chemistry of prions: small molecules, protein conformers and mass spectrometry. Meeting Abstract. Prion (Supplement 1) 9: S70.
Using proteinase K to study the structure of prions.     Reprint Icon - (Abstract Only)
Silva, C.J., Vazquez-Fernandez, E., Requena, J.R. 2015. Using proteinase K to study the structure of prions.. Meeting Abstract. Prion (Supplement 1) 9: S69-S70.
PrP0\0 mice show behavioral abnormalities that suggest PrPC has a role in maintaining the cytoskeleton.     Reprint Icon - (Abstract Only)
Schmitz, M., Silva, C.J. 2015. PrP0\0 mice show behavioral abnormalities that suggest PrPC has a role in maintaining the cytoskeleton.. Meeting Abstract. Prion (Supplement 1) 9: S73-S74..
The view from above: The potential of aerial surveillance in identifying CWD infected herds.     Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2015. The view from above: The potential of aerial surveillance in identifying CWD infected herds.. Meeting Abstract. Prion (Supplement 1) 9: S73..
Detection of shiga toxins by lateral flow assay - (Peer Reviewed Journal)
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. 2015. Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163.
Proteinase K and the structure of PrPse: the good, the bad, and the ugly     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Vázquez-Fernández, E., Onikso, B., Requena, J.R. 2015. Proteinase K and the structure of PrPse: the good, the bad, and the ugly. Virus Research. doi: 10.1016/j.virusres.2015.03.008.
Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. - (Book / Chapter)
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Sample Preparation of Plant, Animal and Soil. In: Springer Press. Eds. M. Micic and Whyte. J.D., editors p.389-399.
Behavioral abnormalities in prion protein knockout mice and the potential relevance of PrPc for the cytoskeleton     Reprint Icon - (Peer Reviewed Journal)
Schmitz, M., Zafar, S., Silva, C.J., Zerr, I. 2014. Behavioral abnormalities in prion protein knockout mice and the potential relevance of PrPc for the cytoskeleton. Prion. 8(6) 381-386.
A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays - (Book / Chapter)
Stanker, L.H., Hnasko, R.M. 2015. A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays. Methods in Molecular Biology. Springer Science and Business Media, New York. 216 pp. 2015. (Book Chapter.
The generation of monoclonal antibodies and their use in rapid diagnostic tests - (Proceedings)
Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A., Lin, A.V. 2014. The generation of monoclonal antibodies and their use in rapid diagnostic tests. Proceedings: Microfluidics Regenerated: New Approaches, Applications and Enabling Technologies. Meeting Abstract. 7(1-16).
Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
Overview of the Western Regional Research Center, Albany, California.     Reprint Icon - (Other)
Silva, C.J. 2014. Overview of the Western Regional Research Center, Albany, California. Meeting Proceedings. #10.
Oxidized methionine is not a prion-specific covalent modification     Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I., Hui, C., Carter, J.M. 2014. Oxidized methionine is not a prion-specific covalent modification. Meeting Abstract. Abstracts of the Papers of the American Chemical Society. 248: 230-BIOL.
The physiological role of the normal cellular prion protein (PrPC).     Reprint Icon - (Abstract Only)
Schmitz, M., Greis, C., Ottis, P., Silva, C.J., Schulz-Schaeffer, W.J., Wrede, A., Koppe, K., Requean, J.R. 2014. The physiological role of the normal cellular prion protein (PrPC) [abstract]. Meeting Abstract. Current Topics in Biological Chemistry. Paper No. 113.
Small molecules and antibodies: a means of distinguishing between PrPC and PrPSc     Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A. 2014. Small molecules and antibodies: a means of distinguishing between PrPC and PrPSc [abstract]. Meeting Abstract. Current Topics in Biological Chemistry. Paper No. 12.
Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range.     Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure     Reprint Icon - (Peer Reviewed Journal)
Silva, C.J. 2014. Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure. Prion. 8(1):42-50. DOI: http://dx.doi.org/10.4161/pri.27891.
Distinguishing between PrPC and PrPSc using small molecule reagents - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Dynin, I., Carter, J.M. 2013. Distinguishing between PrPC and PrPSc using small molecule reagents. Meeting Abstract. Prion 2013 Banff, Alberta, Canada May 26-29, 2013. jdc..
Detection of bacterial toxins by lateral flow immunoassay - (Proceedings)
Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of bacterial toxins by lateral flow immunoassay. p. 1-28. Irvine, CA; Biodot.
Oxidation of methionine in PrP is dependent upon the oxidant and the amino acid two positions removed - (Abstract Only)
(01-Oct-12)
Last Modified: 7/25/2016
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