Novel Yield Genes from Cultivated and Wild Japanese Soybean in North Carolina
Soybean and Nitrogen Fixation Research
2013 Annual Report
1a.Objectives (from AD-416):
Identify yield genes in soybean.
1b.Approach (from AD-416):
Molecular techniques which identify and tag yield genes offer the opportunity to advance soybean yields. However, this new technology produces agricultural success only when applied to the appropriate genetic breeding materials. The authors of this proposal have developed unique genetic materials, derived from Japan, that are ideally suited to identifying and tagging yield genes. The essence of this proposal is to: i) use our new soybean breeding materials as test subjects to integrate next generation molecular technology with field breeding, ii) provide proof of concept for the synergy of these two disciplines, and iii) develop genetic products that can be utilized in the soybean industry.
This project is related to Objective 1 of this in-house project: to discover novel genes/alleles in soybean for ‘improved yield potential’, determine their inheritance, determine genomic location, transfer to adapted germplasm, and release. Soybean is among the least diverse crops in the USA, as it is now grown on the farm. Such narrow genetic diversity limits the ability of breeders to improve the crop and add value for the consumer. New genetics are needed to address this diversity problem. This project examines agronomic genetic diversity derived from wild soybean. Three putative yield genes were identified in wild soybean based on previous work in our unit. In the present research, we developed near isogenic lines (NILs) which are polymorphic at these loci and are conducting validation yield trials to determine the impact of these loci. We are also seeking to discover the presence of yield-promoting alleles from wild soybean through studies of heterosis. The parental stocks for this research were seventeen adapted erect inbred breeding lines previously derived from a hybridization of cultivated soybean x wild soybean (N7103 x PI 3366122). These 17 were backcrossed to the cultivated parent and F2 bulk populations were tested for heterosis. Two of the 17 populations exhibited substantial heterosis for yield in 2012 over four environments and are being retested for yield in 2013.
The Authorized Departmental Officer's Designated Representative monitored activities of project through frequent phone calls and emails, two formal meetings, site visits, and through quarterly reports.