2013 Annual Report
1a.Objectives (from AD-416):
This research project seeks determine the efficacy of chemical disinfectants against foreign animal disease (FAD) viruses dried on surfaces relevant to the pork packing industry. Specific objectives are:
1. Develop surface disinfection methodologies.
2. Once methodologies are validated for use with standardized chemical disinfectants, commercial disinfectants identified by pork producers will be tested in assays.
3. Identify potential surrogates for Foot-and-Mouth Disease virus, African Swine Fever virus and Classical Swine Fever virus to do side-by-side comparison assays to determine the efficacy with each FAD virus/surrogate pair. Once validated, these surrogates can be used outside the high containment laboratories.
1b.Approach (from AD-416):
Development of surface disinfection methodologies would be variable due to surface properties (metal, concrete, rubber) and viral consentration and temperature. Foreign animal disease (FAD) viruses Foot-and-Mouth Disease (FMD), Classical Swine Fever (CSF) and African Swine Fever (ASF) would be used as model agents, dried on stainless steel, plastic and wood surfaces and disinfection efficacy of selected chemicals determined. Data will be generated for dose-response, timecourse and requirements for completion of disinfection.
Once the above methodologies have been determined for use with standardized chemical disinfectants, commercial disinfectants identified by pork producers will be tested in the assays. Once an effective neutralization plan is developed for the targed disinfectants, efficacy data will be generated for each disinfectant on all three viruses on dried surfaces.
Potential surrogates for FMDV, ASFV and CSFV will be identified in collaboration with the pork board. Side-by-side comparative disinfectant efficacy tests will be conducted with each FAD virus/surrogate pair. Once validated, these surrogates can be used outside high containment as needed to test newly formulated disinfectants.
Activities conducted for the project, which was initiated in FY 2013, include.
1)determining FAD virus stability in swine blood and feces before and after drying;.
2)developing a method for FAD virus disinfection in blood and feces.
3)determining the inhibition by blood and feces of citric acid and bleach disinfection on stainless steel surfaces. For staff, we selected an ORISE summer student to work on the project, who was highly successful at generating disinfection and stability data. The initial phases of the project are almost complete.
No technologies were transferred or publications produced during this reporting period.