Project Number: 1940-32000-057-46
Project Type: Trust

Start Date: Dec 15, 2012
End Date: Dec 15, 2014

Objective:
This research project seeks determine the efficacy of chemical disinfectants against foreign animal disease (FAD) viruses dried on surfaces relevant to the pork packing industry. Specific objectives are: 1. Develop surface disinfection methodologies. 2. Once methodologies are validated for use with standardized chemical disinfectants, commercial disinfectants identified by pork producers will be tested in assays. 3. Identify potential surrogates for Foot-and-Mouth Disease virus, African Swine Fever virus and Classical Swine Fever virus to do side-by-side comparison assays to determine the efficacy with each FAD virus/surrogate pair. Once validated, these surrogates can be used outside the high containment laboratories.

Approach:
Development of surface disinfection methodologies would be variable due to surface properties (metal, concrete, rubber) and viral consentration and temperature. Foreign animal disease (FAD) viruses Foot-and-Mouth Disease (FMD), Classical Swine Fever (CSF) and African Swine Fever (ASF) would be used as model agents, dried on stainless steel, plastic and wood surfaces and disinfection efficacy of selected chemicals determined. Data will be generated for dose-response, timecourse and requirements for completion of disinfection. Once the above methodologies have been determined for use with standardized chemical disinfectants, commercial disinfectants identified by pork producers will be tested in the assays. Once an effective neutralization plan is developed for the targed disinfectants, efficacy data will be generated for each disinfectant on all three viruses on dried surfaces. Potential surrogates for FMDV, ASFV and CSFV will be identified in collaboration with the pork board. Side-by-side comparative disinfectant efficacy tests will be conducted with each FAD virus/surrogate pair. Once validated, these surrogates can be used outside high containment as needed to test newly formulated disinfectants.