1a.Objectives (from AD-416):
The objective of this research is to determine levels of PRRS viral RNA in porcine tonsils of PHGC pigs to assess PRRS persistence. To assess tonsil tissue RNA for immune correlates of PRRSV persistence and to expand the capacity of the PHGC database and improve public/private interface. ARS will provide funds to the ISU cooperator for their efforts for the last objective to update the PHGC database and website.
1b.Approach (from AD-416):
ARS has led the PRRS Host Genetics Consortium (PHGC) project to identify genetic determinants of resistance/susceptibility of commercial swine to porcine respiratory and the reproductive syndrome virus (PRRSV) infection and associated growth effects. ARS will use National Pork Board funds to perform research on PRRS viral persistence and immune factors correlated with long term viral persistence. The Iowa State Univ. cooperator has expertise in maintaining relational databases for the National Animal Genome Research Program termed NRSP8.
This new agreement focuses on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the most economically important swine production disease worldwide. As part of the PRRS Host Genetics Consortium (PHGC), a secure, password protected PHGC relational database has been developed: http://www.animalgenome.org/lunney/index.php. To date, data from 14 PHGC trials have been added to the database. These included pedigree, weight, viral load, and genotype data. For this grant ARS scientists at the Beltsville Agricultural Research Center (BARC) in Beltsville, Maryland are working with Iowa State University (ISU) to update the PHGC database. It started with a redesign of the website to make data access more user-friendly, enhance search functions, and improve the overall look of the website. Additional functions added to the website include a page that is designed to handle PowerPoint slide presentations made by PHGC members. Other materials include secure sites for pre-publication sharing of abstracts and articles, as well as public news sites.