Location: Genetics, Breeding, & Animal Health
Project Number: 5438-32000-031-03
Start Date: Oct 01, 2012
End Date: Sep 30, 2017
Assays for approximately 100 single nucleotide polymorphisms (SNPs) will be designed from USMARC ovine DNA sequence information and publicly available information from more than 3000 sheep from 74 breeds around the world. Novel SNP assays will be developed for a matrix-assisted laser desorption/ionization--time-of-flight mass spectrometry (MALDI-TOF MS) platform. The accuracy, reproducibility, and power of these SNPs will be evaluated with USMARC reference DNA samples that have been previously sequenced to determine their genotypes.