Improved Diagnostics and Quantification Assays for Verticillium Species Important to California Agriculture
Crop Improvement and Protection Research
2013 Annual Report
1a.Objectives (from AD-416):
The objective of the project is to further develop molecular diagnostic techniques we are currently working on to broaden their utility to the regulatory and diagnostic infrastructure in California and else where in the USA.
1b.Approach (from AD-416):
We have developed a real time PCR molecular diagnostic procedure for quantification of Verticillium dahliae in the soil that is highly accurate and have preliminary data on a diagnostic marker system for two additional Verticillium spp. The objective of this project is to validate the marker system for the other two species by comparing the results of the molecular assay with traditional soil plating as well as expand the sequence database we have to include marker development for additional species.
This agreement was established in support of objective 2 of the parent project, the goal being to develop molecular diagnostic tools for the identificatioin of emerging diseases of vegetables and strawberry, and use these tools in the development of management strategies as alternatives to methyl bromide. Additional cultures representing Verticillium species of interest to California have been provided to our collaborator and we have been looking at the design of additional TaqMan probes to include these species in the diagnostic assay.